• 메인
  • >
  • 소식
  • >
  • 공지사항
* 일시/장소  : 4월 19일(월) 오후 2시~ , 자연과학동(E6) 2층세미나실 2502호  



Title : In Vivo Fluorescence Laser-Scanning Endomicroscopy for Biomedical Research




Small animal, particularly mouse, has been an important test bed for basic and translational biomedical study preceding clinical application. Recent advances in genomic technology have allowed a creation of animal model for human disease with genetically encoded biomarker, notably green fluorescent protein (GFP) awarded Nobel Prize at 2008. Combined with a rapid development of fluorescent probes, including newly emerging nano-material, and mature molecular biology tools, it has opened up new avenue to the observation of complex pathophysiology of human disease in animal model with much greater detail at cellular and molecular level in high contrast and specificity. Accordingly, novel fluorescence imaging methods that can visualize anatomical structure with functional and molecular information provided by fluorescent probes in animal model in vivo have drawn great attentions. While all of major clinical imaging modality such as ultrasound, CT, MRI and PET has been modified and adapted, optical imaging technologies, especially laser scanning confocal and multiphoton fluorescence microscopy, are the only one readily providing cellular resolution of sub-micrometer in live animal. Over the recent years, these technologies enabled dynamic 3D view of the living specimen as various biological processes unfold in real-time to provide unprecedented insights those were impossible to obtain by traditional static 2D snapshots (i.e. histopathology). With the development of highly specific targeting agents and new endogenous contrast, potentially enabled by nano-technology, these new imaging methods have advanced beyond conventional ex vivo thin tissue sections or in vitro cell cultures in Petri dish to in vivo animal. It can allow direct visualization of gene expression, regulation, protein activity, drug delivery, cell trafficking, cell interaction, physiological response under external stimuli in their natural microenvironment in vivo. Various dynamic processes such as cell trafficking, cell-cell or cell-microenvironment interaction, protein and gene expression can now be monitored in live animal, which provides new insights unobtainable by conventional ex vivo and in-vitro study. However, for in-vivo imaging of many viscera and thoracic organs deeply placed inside animal, application of these techniques has been challenging because of limited imaging depth up to 500 mm.

In this presentation, the recent development of endoscopic method to conduct these in-vivo microscopy techniques in minimally invasive way to visualize deep internal organ and tissue in small animal will be introduced. Fabrication of 1mm diameter miniature gradient index lens-based endomicroscope and its integration to home-built video-rate confocal / multi-photon microscopy system will be presented. Its application to various basic and translational biomedical studies through wide-area cellular imaging of internal organ will be demonstrated1. Specifically, the longitudinal visualization result of immune cell trafficking from the graft to host and vice versa after transplantation in mouse model will be presented. And recent efforts on the visualization of early tumorigenesis of orthotopic spontaneous colorectal cancer mouse model will be presented. Not only for basic biomedical research, this live animal imaging system can serve as a platform for translational research to monitor efficacy of various preclinical therapy, which can greatly expedite clinical translation. With proper engineering and further development of technology, it can evolve to a novel diagnostic tool in certain areas of everyday human clinic with new nano-engineered materials.

1.     Kim, P et. al. “In vivo wide-area cellular imaging by side-view endomicroscopy,” Nature Methods, 2010. 7(4), pp. 303-305



나노과학기술대학원 책임교수 신중훈 배상.

번호 제목 날짜 조회 수
공지 물리학과 교수초빙 공고 2017.12.01 819
공지 홈페이지 게시글을 핸드폰 등으로 쉽게 확인하는 법 2015.10.27 2598
454 장학금 관련 안내는 portal의 "학생공지사항"->"게시판"->생활관,장학,복지를 이용하세요. 2009.02.01 255969
453 학위논문심사일정 및 작성요령 (Guidelines on the Completion and Submission of Master’s and Doctoral Dissertations) file 2010.03.15 220295
452 세미나공지-김용운박사(KIAS) 4월12일(월)오전11시~,자연과학동 2502호 2010.04.07 81085
451 [학사과정] '물리학과 전공교과목 및 연구학점' 이수 빈번한 오류(Caution on research course for graduation, Undergraduates) file 2014.03.04 80407
450 [대학원] 교과목 이수 요건 file 2014.11.24 73534
449 [최종공지]Special Invited Seminar on “The manuscript selection process at SCIENCE" 2010.04.01 70799
» 세미나공지-김필한박사(Harvard Univ.) 4월19일(월)오후2시~,자연과학동 2502호 2010.04.15 69429
447 콜로키움 일정변경 : 11월 30일자 -> 12월 7일로 변경됨, 11월 30일은 콜로키움 없습니다. 2009.11.12 69026
446 2010 대학원 신입생 등록 안내 file 2009.10.29 68459
445 미국무부 초청 연사 강연 (7월 10일) 알림 file 2009.07.09 68144
444 이투힐 양희영 장학생 선발 안내 file 2010.03.26 66179
443 Call for Proposals on On-Line Electric Vehicle (OLEV) file 2010.04.02 65631
442 2010학년도 후기 대학원 신입생 선발 모집요강 file 2010.03.25 64762
441 [대학원] 자격시험 안내 (대학원 신입생들은 반드시 숙지하세요) 2014.11.24 62310
440 홍콩과기대 교환학생 신청 - 3월 24일까지 file 2009.03.20 62000
439 [대학원][학부] 2015년 2월 및 8월 졸업대상자 학위논문제출일정 및 작성요령 안내 file 2014.11.24 61452
438 [대학원] 박사 예심 안내 file 2014.11.24 61200
437 Call for HRHR Proposals on New and Non-Conventional Hydrogen Generation Schemes file 2009.08.14 61166
436 [KEPSI]하이닉스반도체 "산학장학생 리쿠르팅 및 선발설명회" 2010.04.06 60767
435 윤리 및 안전 교과목 이수 안내 - 전학생(학부 및 대학원생) 반드시 이수해야 합니다. file 2009.03.05 60689